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目的了解宁夏部分地区蚊虫种类组成和不同蚊种细胞色素氧化酶亚基Ⅰ(COI)基因特征和蚊虫分子系统进化关系。方法对宁夏银川市、石嘴山市、吴忠市和青铜峡市的三带喙库蚊、里海伊蚊、淡色库蚊和八代按蚊4种蚊种COI基因序列进行扩增、测序,在Gen Bank中对所测序列进行相似性比对,分析基因特征、颠换率、遗传距离,利用Mega 7.0软件构建分子系统树。结果本研究中相似性比对结果,其他蚊虫分别与其同种蚊虫的相似性均达到94%99%,COI基因序列扩增长度为417 bp左右,GC含量为29.4%31.5%,种间颠换率为5.80%13.81%,种间遗传距离为0.1210.160。结论 COI基因具有种间特异性,可作为不同蚊种分类鉴别的参考依据。
Abstract：Objective To investigate the relationship between cytochrome oxidase subunit Ⅰ( COI) gene traits and molecular evolution of mosquitoes from parts of area in Ningxia. Methods COI genes of four species of mosquitoes including Culex tritaeniorhynchus,Aedes caspius,Culex pipiens pallens and Anopheles yatsushiroensis from Yinchuan,Shi Zuishan,Wuzhong and Qingtongxia were amplified,sequenced and blasted in Gen Bank. Gene traits,transversion rates and genetic distance were analyzed.Molecular phylogenetic trees were generated using Mega 7. 0. Results COI genes studied were about 417 bp with GC contents from 29. 4% to 31. 5%,transversion rates from 5. 80% to 13. 81% and genetic distances from 0. 121 to 0. 160 among species. Conclusion COI gene is of species specificity,and can be used for the identification basis of different mosquitoes.
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基本信息:
DOI：10.19821/j.1671-2781.2017.04.024
中图分类号:R384.1
引用信息:
[1]韩坤,吴忠兰,兰策介等.宁夏部分地区不同蚊种细胞色素氧化酶亚基Ⅰ(COI)基因序列分析[J].中华卫生杀虫药械,2017,23(04):373-375.DOI:10.19821/j.1671-2781.2017.04.024.
基金信息:
宁夏自然科学基金项目(编号:NZ14227)