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传统的测序技术得到的是数以万计细胞平均后的变异水平,而单细胞测序技术从单细胞层面上对细胞基因组或转录组进行分析,使生物研究达到了前所未有的分辨率和规模,为生命科学研究提供了新的视角与方法。本文简要总结了单细胞分离技术,回顾了各种单细胞技术及其在当前各领域中的应用,展望了单细胞测序技术未来发展的潜在机会。
Abstract：Traditional sequencing technologies obtain the level of variation after averaging tens of thousands of cells, yet single-cell sequencing technologies analyze a cell's genome or transcriptome at the single-cell level, leading to an unprecedented resolution and scale of biological research and providing new perspectives and methods for life science research.This paper briefly summarizes single-cell isolation technologies, reviews various single-cell technologies and their applications in various fields at present, and outlooks potential opportunities for the future development of single-cell sequencing technologies.
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基本信息:
DOI：10.19821/j.1671-2781.2021.03.024
中图分类号:R318
引用信息:
[1]路大峰,罗正汉,汪春晖.单细胞测序技术原理及其应用研究进展[J].中华卫生杀虫药械,2021,27(03):280-284.DOI:10.19821/j.1671-2781.2021.03.024.
基金信息:
全军军事理论科研项目第二批(编号：CLB19J027)